Effects of ganglioside GM1 on the thermotropic behavior of cholera toxin B subunit

@article{Dalziel1984EffectsOG,
  title={Effects of ganglioside GM1 on the thermotropic behavior of cholera toxin B subunit},
  author={Adam W. Dalziel and Gert Lipka and Babur Z. Chowdhry and Julian M. Sturtevant and David E. Schafer},
  journal={Molecular and Cellular Biochemistry},
  year={1984},
  volume={63},
  pages={83-91}
}
The B, or binding, subunit of cholera enterotoxin forms a pentameric ring structure in the intact toxin, and also when the subunit is isolated from the A subunit. The thermal denaturation of the B subunit ring was examined by differential scanning calorimetry in the presence and absence of ganglioside GM1, its natural ‘receptor’. In the absence of ganglioside an irreversible endotherm was observed with maximal excess apparent heat capacity, Cmax, at 74.6° C. When the ganglioside was added in… CONTINUE READING