Effects of free fatty acids on the organization of cytoskeletal elements in lymphocytes.

  title={Effects of free fatty acids on the organization of cytoskeletal elements in lymphocytes.},
  author={Richard L. Hoover and Ken Fujiwara and Richard D. Klausner and Deepak K. Bhalla and Robert W. Tucker and Morris J. Karnovsky},
  journal={Molecular and cellular biology},
  volume={1 10},
Treatment of mouse lymphocytes with cis-unsaturated free fatty acids produced alterations in the immunofluorescence patterns of the cytoskeleton and contractile proteins. Saturated free fatty acids and trans-unsaturated free fatty acids had no effect. In untreated cells, the microtubular pattern exhibited radiation from an organizing center, resembling the spokes of an umbrella. The addition of linoleic acid produced a polarized submembranous aggregate. Under control conditions, staining for… Expand
The effect of free fatty acids on spectrin organization in lymphocytes
The hypothesis that cytoskeletal reorganization in lymphocytes may be related to the altered immune function frequently observed in these conditions is supported. Expand
Lipid alterations in LLC-PK1 cells exposed to mercuric chloride.
Treatment of cells with HgCl2 caused a rapid accumulation of unesterified fatty acids (particularly arachidonic acid) and lysophospholipids and the formation of blebs on the plasma membrane and irreversible injury as indicated by electron microscopy. Expand
Effect of saturated and unsaturated fatty acids on the oxidative metabolism of human neutrophils. The role of calcium ion in the extracellular medium.
Results indicate that the inhibitory effect of Ca2+ on the fatty acid-induced O2- generation might be due to the ionic interaction between the carboxyl group of the fatty acids and Ca2+. Expand
Role of cholesterol in the capping of surface immunoglobulin receptors on murine lymphocytes
A model is proposed in which the protein(s) involved in capping is located in a gel-like lipid domain, and that removal of cholesterol makes this domain less gel-Like and inhibits capping, and Restoration of the gel- like nature of this domain by the addition of either cholesterol or stearic acid enables the protein to function normally. Expand
The effect of hydroxylated fatty acid-containing phospholipids in the remodeling of lipid membranes.
The synthetic fatty acid 2-hydroxyoleic acid (2OHOA) is an antitumor drug that regulates membrane lipid composition and structure. An important effect of this drug is the restoration of sphingomyelinExpand
Localization of phospholipase A2 in normal and ras-transformed cells
The cellular localization of phospholipase A2 was examined in normal and ras-transformed rat fibroblasts using immunohistochemical techniques and indicated a spatial proximity between PLA2 and rAs proteins in the ruffles of ras -transformed cells. Expand
Polyunsaturated fatty acids induce polarized submembranous F‐actin aggregates and kill Entamoeba histolytica
It is found that α‐linolenic acid disrupts the actin cytoskeletal network, which led to the detachment of plasma membrane from the underlying cytoskeleton and showed accumulation of long and numerous filopodia at the cap site. Expand
Labeling of Phospholipids and Preparation of Cell Membranes for P L A 2 Assay Subcontinent
Abs trac t . The cellular localization of phospholipase A2 (PLA2) was examined in normal and ras-transformed rat fibroblasts using immunohistochemical techniques. Polyclonal antibodies were generatedExpand
Effects of linoleic acid and mitogenic stimulation on the fatty acid composition of human lymphocytes.
It is suggested that profound alterations of membrane fatty acid composition occur spontaneously during the mitotic cycle, and may be further induced by experimental manipulation, without gross perturbation of cell function. Expand
The cellular fatty acid binding proteins: aspects of structure, regulation, and function.
  • N. Bass
  • Biology, Medicine
  • International review of cytology
  • 1988
Publisher Summary This chapter provides a comprehensive overview of the current state of knowledge of the research with particular emphasis on evolving concepts of fatty acid binding proteins (FABP)Expand