Effects of free fatty acids on somatostatin secretion, content and mRNA levels in cortical and hypothalamic fetal rat neurones in monolayer culture.

@article{Sears1993EffectsOF,
  title={Effects of free fatty acids on somatostatin secretion, content and mRNA levels in cortical and hypothalamic fetal rat neurones in monolayer culture.},
  author={Rosa Se{\~n}ar{\'i}s and Mark David Lewis and Francisca Lago and Fernando Dom{\'i}nguez and Maurice F. Scanlon and Carlos Di{\'e}guez},
  journal={Journal of molecular endocrinology},
  year={1993},
  volume={10 2},
  pages={207-14}
}
The effects of free fatty acids on somatostatin secretion, content and mRNA levels in fetal rat hypothalamic and cortical cell cultures were investigated. Somatostatin secretion and content were quantified by radioimmunoassay. Somatostatin mRNA levels were measured by Northern blot hybridization using a cRNA probe. Treatment with either caprylic acid (5 x 10(-3) M) or oleic acid (5 x 10(-5) M) for 90 min inhibited basal somatostatin secretion in both hypothalamic and cortical cell cultures. In… CONTINUE READING