Effects of fasting and insulin administration on polyribosome formation in rat epididymal fat cells.

@article{Lyons1980EffectsOF,
  title={Effects of fasting and insulin administration on polyribosome formation in rat epididymal fat cells.},
  author={Russet te M. Lyons and Steven K. Nordeen and David A. Young},
  journal={The Journal of biological chemistry},
  year={1980},
  volume={255 13},
  pages={6330-4}
}
Polyribosomes from rat epididymal fat pads may be prepared in a synthetically active state and high yield by quick-freezing the tissue in liquid nitrogen, followed by grinding to a frozen powder. The number of initiated (active) ribosomes is limiting in a cell-free protein synthesis assay derived from the frozen preparation that measures completion of nascent polypeptide chains. When starved rats are killed 30 min after a single injection of insulin (0.8 unit, subcutaneously) there is a shift… CONTINUE READING