Effects of extender, incubation temperature, and added seminal plasma on capacitation of cryopreserved, thawed boar sperm as determined by chlortetracycline staining.

@article{Vadnais2005EffectsOE,
  title={Effects of extender, incubation temperature, and added seminal plasma on capacitation of cryopreserved, thawed boar sperm as determined by chlortetracycline staining.},
  author={Melissa L Vadnais and Roy Neville Kirkwood and David J Specher and Karen C. Chou},
  journal={Animal reproduction science},
  year={2005},
  volume={90 3-4},
  pages={347-54}
}
The effect on apparent capacitation status of frozen-thawed (FT), washed boar sperm was examined in capacitation-supporting medium at 39 degrees C without added seminal plasma (SP) or supplemented with either 10 or 20% (v/v) boar SP. The thawed sperm from three boars were washed to remove the egg yolk-based freezing medium (EY) and then incubated for 1-8h after addition of SP. Capacitation status of the sperm was determined microscopically using chlortetracycline staining patterns. At 1h after… CONTINUE READING
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