Effects of estrogenic xenobiotics on human and mouse spermatozoa.

  title={Effects of estrogenic xenobiotics on human and mouse spermatozoa.},
  author={Lynn R. Fraser and Ergin Beyret and Stuart R. Milligan and Susan A Adeoya-Osiguwa},
  journal={Human reproduction},
  volume={21 5},
OBJECTIVE To investigate human sperm responsiveness to the estrogenic xenobiotic genistein and seek further information regarding the mechanism of action of estrogenic xenobiotics using mouse spermatozoa. METHODS Uncapacitated human spermatozoa were incubated with genistein and assessed using chlortetracycline (CTC) fluorescence. CTC was also used to evaluate mouse sperm responses to daidzein and combinations of genistein, 8-prenylnaringenin and nonylphenol. Several steroids were tested to… CONTINUE READING