• Corpus ID: 1138258

Effects of electropermeabilization and gene electrotransfert on the lateral mobility of a GPi anchored protein

@article{Escoffre2012EffectsOE,
  title={Effects of electropermeabilization and gene electrotransfert on the lateral mobility of a GPi anchored protein},
  author={Jean-Michel Escoffre and M. Hubert and Justin Teissi'e and Marie-Pierre Rols and Cyril Favard},
  journal={arXiv: Biomolecules},
  year={2012}
}

• J. Escoffre, M. Hubert, +2 authors C. Favard
• Published 7 February 2012
• Biology
• arXiv: Biomolecules

Electropermeabilization is a method by which molecules can be introduced in living cells through the plasma membrane. Here, we show that electropermeabilization affects the lateral mobility of Rae-1, a GPi anchored protein. Our results shows that 10 to 20 % of the membrane surface is occupied by defaults or pores. Gene electrotransfert also affects the lateral mobility of Rae-1. Furthermore, we clearly show that once inserted into the plasma membrane, DNA totaly excludes Rae-1, indicating that DNA molecules are tightly packed together to forms huge aggregates.