Effects of either glucose or fructose and metabolic regulators on bovine embryo development and lipid accumulation in vitro.

@article{BarcelFimbres2007EffectsOE,
  title={Effects of either glucose or fructose and metabolic regulators on bovine embryo development and lipid accumulation in vitro.},
  author={M Barcel{\'o}-Fimbres and George E. Seidel},
  journal={Molecular reproduction and development},
  year={2007},
  volume={74 11},
  pages={1406-18}
}
Our objective was to determine if replacing glucose with fructose would decrease cytoplasmic lipid accumulation during culture of embryos with or without regulators of metabolism. In vitro-produced bovine zygotes were cultured 60 hr in chemically defined medium-1 (CDM-1) plus 0.5% BSA and 0.5 mM fructose or glucose in Experiment 1, and glucose in Experiment 2. In both experiments, 8-cell embryos were next cultured 135 hr in CDM-2 plus 2 mM fructose or glucose in factorial combination with five… CONTINUE READING

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