Effects of duration, concentration, and timing of ionomycin and 6-dimethylaminopurine (6-DMAP) treatment on activation of goat oocytes.

@article{Lan2005EffectsOD,
  title={Effects of duration, concentration, and timing of ionomycin and 6-dimethylaminopurine (6-DMAP) treatment on activation of goat oocytes.},
  author={G. Zhiyong Lan and Dong Han and Yan-Guanng Wu and Zheng-Bin Han and Suo-Feng Ma and Xin-yong Liu and Chong-Le Chang and Jing-He Tan},
  journal={Molecular reproduction and development},
  year={2005},
  volume={71 3},
  pages={
          380-8
        }
}
The protocol of ionomycin followed by 6-dimethylaminopurine (6-DMAP) is commonly used for activation of oocytes and reconstituted embryos. Since numerous abnormalities and impaired development were observed when oocytes were activated with 6-DMAP, this protocol needs optimization. Effects of concentration and treatment duration of both drugs on activation and development of goat oocytes were examined in this study. The best oocyte activation (87-95%), assessed by pronuclear formation, was… CONTINUE READING
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