Effects of disulfide bond reducing agents on sperm chromatin structural integrity and developmental competence of in vitro matured oocytes after intracytoplasmic sperm injection in pigs.

@article{Cheng2009EffectsOD,
  title={Effects of disulfide bond reducing agents on sperm chromatin structural integrity and developmental competence of in vitro matured oocytes after intracytoplasmic sperm injection in pigs.},
  author={Wen-Min Cheng and Lei An and Zhong-Hong Wu and Yu-bo Zhu and Jing-hao Liu and Hong-Mei Gao and X Li and Shi-jun Zheng and Dong-bao Chen and Jian-hui Tian},
  journal={Reproduction},
  year={2009},
  volume={137 4},
  pages={633-43}
}
We recently reported that electrical activation followed by secondary chemical activation greatly enhanced the developmental competence of in vitro matured porcine oocytes fertilized by intracytoplasmic sperm injection (ICSI). We hypothesized that sperm treatment with disulfide bond reducing agents will enhance the development competence of porcine embryos produced by this ICSI procedure. We examined the effects of glutathione (GSH), dithiothreitol (DTT), GSH or DTT in combination with heparin… CONTINUE READING