Effects of dilution procedure and culture conditions after thawing on survival of frozen bovine blastocysts produced in vitro.

@article{Massip1993EffectsOD,
  title={Effects of dilution procedure and culture conditions after thawing on survival of frozen bovine blastocysts produced in vitro.},
  author={Alban Massip and Pascal Mermillod and C Wils and Franz Dessy},
  journal={Journal of reproduction and fertility},
  year={1993},
  volume={97 1},
  pages={65-9}
}
Blastocysts derived from bovine zygotes fertilized and matured in vitro and cultured for 7 days in conditioned medium were frozen in 1.36 mol glycerol l-1 and 0.25 mol sucrose l-1. In vitro survival after thawing was unaffected by dilution rate in 0.25 mol sucrose l-1. The proportion of blastocysts that re-expanded after 24 h was 81% (70 of 86) and 47% (33… CONTINUE READING