Effects of differing oocyte-secreted factors during mouse in vitro maturation on subsequent embryo and fetal development

@article{Sudiman2013EffectsOD,
  title={Effects of differing oocyte-secreted factors during mouse in vitro maturation on subsequent embryo and fetal development},
  author={Jaqueline Sudiman and Lesley J. Ritter and Deanne Kate Feil and Xiao Wang and K T Chan and David G. Mottershead and David M. Robertson and Jeremy G. Thompson and Robert Bruce Gilchrist},
  journal={Journal of Assisted Reproduction and Genetics},
  year={2013},
  volume={31},
  pages={295-306}
}
We hypothesised that varying native oocyte-secreted factor (OSF) exposure or using different recombinant OSF peptides would have differential effects on post-in vitro maturation (IVM) embryo and fetal development. Mouse cumulus oocyte complexes (COCs) were treated with the purified mature domain of GDF9 and/or BMP15 or were co-cultured with denuded oocytes (DOs) from 0 h or 3 h of IVM. DOs were matured for 3 h as either intact COCs+/-FSH before denuding, or as DOs + FSH. COCs were fertilised… CONTINUE READING