[Effects of different factors on direct induction of microtubers from explants in Pinellia ternate].

Abstract

OBJECTIVE To study the effects of different factors on direct induction of microtubers. These factors included plant growth substances, casein hydrolysate (CH), active carbon (AC), polyethylene glycol (PEG 4000), sucrose and glucose. METHOD Using the orthogonal design method. RESULT AND CONCLUSION The optimal media to directly induce microtubers from leaves were MS + MET 0.5 mg x L(-1) + 6-BA 0.5 mg x L(-1) + sucrose 3% and MS+ 6-BA 0.5 mg x L(-1) + IAA 0.5 mg x L(-1) + sucrose 3%. Optimal media to directly induce microtubers from tubers were MS + 6-BA 1.0 mg x L(-1) + PEG 5% + sucrose 5% and MS+ CH 500 mg x L(-1) + MET 0.5 mg x L(-1) + 6-BA 0.5 mg x L(-1) + AC 0.5% + sucrose 5%. Media suitable for plantlet growth were MS + 6-BA 0.5 mg x L(-1) + IAA 0.5 mg x L(-1) + AC 0.5% + sucrose 5% and MS + MET 0.5 mg x L(-1) + 6-BA 0.5 mg x L(-1) + sucrose 3%.

Cite this paper

@article{Zhang2005EffectsOD, title={[Effects of different factors on direct induction of microtubers from explants in Pinellia ternate].}, author={Ai-min Zhang and Sheng-Yu Yang and Jianping Xue and Wei Sheng and Xiang Gao and Yan-fang Zhu}, journal={Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica}, year={2005}, volume={30 8}, pages={576-9} }