Effects of deoxyribonucleotide substitutions in the substrate strand on hammerhead ribozyme-catalyzed reactions.

Abstract

In order to examine the effects of deoxyribonucleotide substitutions in the substrate strand, several chimeric DNA/RNA substrates for a hammerhead ribozyme were chemically synthesized. Measurements of kinetic parameters revealed that a chimeric DNA/RNA substrate, that contained GUC at the cleavage site as ribonucleotides, was cleaved by an all-RNA ribozyme… (More)

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Cite this paper

@article{Shimayama1994EffectsOD, title={Effects of deoxyribonucleotide substitutions in the substrate strand on hammerhead ribozyme-catalyzed reactions.}, author={Takashi Shimayama}, journal={Gene}, year={1994}, volume={149 1}, pages={41-6} }