Genetic characterisation and fine mapping of a chlorophyll-deficient mutant (BnaC.ygl) in Brassica napus
Chloroplast development and chlorophyll biosynthesis are co-regulated. Treatment by levulinic acid resulted in a linear relation in both chlorophyll and carotenoid contents, during greening of etiolated French bean leaf discs. Chlorophyll biosynthesis appeared to control that of caroteins. In the presence of levulinic acid; at different levels, photosystem II (PS II) activity decreased when expressed on a chlorophyll basis. Chlorophyllase activity was increased progressively by increasing levulinic acid concentration. Thus, levulinic acid could be used to arrest the light-induced chloroplast development at a desired phase of greening and acts as determinator of chloroplast development in green tissues.