Effects of cryoprotectant concentration and cooling rate on vitrification of aqueous solutions

  title={Effects of cryoprotectant concentration and cooling rate on vitrification of aqueous solutions},
  author={Viatcheslav Berejnov and Naji S. Husseini and Osama A Alsaied and Robert E. Thorne},
  journal={Journal of Applied Crystallography},
Vitrification of aqueous cryoprotectant mixtures is essential in cryopreservation of proteins and other biological samples. Systematic measurements of critical cryoprotective agent (CPA) concentrations required for vitrification during plunge-cooling from T = 295 K to T = 77 K in liquid nitrogen are reported. Measurements on fourteen common CPAs, including alcohols (glycerol, methanol, 2-propanol), sugars (sucrose, xylitol, dextrose, trehalose), polyethylene glycols (ethylene glycol, PEG 200… Expand

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