Effects of cryopreservation on the intracellular calcium concentration of human spermatozoa and its response to progesterone

@article{McLaughlin1994EffectsOC,
  title={Effects of cryopreservation on the intracellular calcium concentration of human spermatozoa and its response to progesterone},
  author={Eileen A. McLaughlin and William Christopher Liberty Ford},
  journal={Molecular Reproduction and Development},
  year={1994},
  volume={37}
}
The intracellular free calcium concentration [Ca2+]i of sperm from 23 ejaculates was measured before and after cryopreservation using the fluorescent probe Fura‐2. Spermatozoa were treated with 3.18 μM progesterone so that the regulation of [Ca2+]i in a dynamic situation could be studied. [Ca2+]i (nM) was 290 ± 13 in fresh spermatozoa vs. 550 ± 26 in cryopreserved samples (mean ± S.E.M. P < 0.0001 paired t‐test). Progesterone at a dose of 3.18 μM stimulated a large and rapid increase in [Ca2+]i… 
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Effects of cryopreservation on Ca2+ signals induced by membrane depolarization, caffeine, thapsigargin and progesterone in boar spermatozoa.
TLDR
Cryopreservation reduces the responsiveness of spermatozoa to depolarization, modulators of the internal Ca2+ store and progesterone in terms of the Ca2+.
Effects of cryo-injury on progesterone receptor(s) of canine spermatozoa and its response to progesterone.
Effects of Ca-ATPase inhibitors on the intracellular calcium activity and motility of human spermatozoa.
TLDR
The results are consistent with high [Ca2+]i in the range observed in caput epididymal or cryopreserved spermatozoa inhibiting motility, but might be confounded by other events following the acrosome reaction.
Assessment of intracellular Ca2+, cAMP and 1,2-diacylglycerol in cryopreserved buffalo (Bubalus bubalis) spermatozoa on supplementation of taurine and trehalose in the extender.
TLDR
Levels of intracellular calcium, cAMP and DAG in fresh and frozen-thawed buffalo spermatozoa cryopreserved in the presence and absence of taurine or trehalose clearly indicated the reduced level of capacitation-like changes in buffalo spermutozoa.
Effect of exogenous progesterone on post-thaw capacitation and acrosome reaction of bovine spermatozoa.
TLDR
The results demonstrate that in cryopreserved bovine semen, P4 acts as capacitating, but not as an AR-inducing agent.
Progesterone-mediated calcium influx and acrosome reaction of human spermatozoa: pharmacological investigation of T-type calcium channels.
The mechanisms of the progesterone (P4)-activated Ca2+ influx and the relationship between the intracellular free Ca2+ concentration ([Ca2+]i) and the acrosome reaction (AR) were investigated in this…
The effect of progesterone on motility and the acrosome reaction in vitrified and cryopreserved human spermatozoa
TLDR
The progesterone treatment after cryopreservation was able to significantly improve the motility parameters of sperm samples and it had an especially large impact on samples classed as teratozoospermic.
Nifedipine reveals the existence of two discrete components of the progesterone-induced [Ca2+]i transient in human spermatozoa.
The motility of demembranated human spermatozoa is inhibited by free calcium ion activities of 500 nmol/L or more.
TLDR
Calcium ion activities of > or =500 nmol/L significantly inhibited the percentage of demembranated-reactivated spermatozoa that were motile, and the velocity and lateral head displacement of these cells, suggesting that calcium ion activities in the range observed in cryopreserved spermatozosa can inhibit the activity of demEMbranation-reactivation human spermatoza.
Gonadotropin-Releasing Hormone-Stimulated Sperm Binding to the Human Zona Is Mediated by a Calcium Influx1
TLDR
The results suggest that GnRH increases sperm-zona binding via an elevation of [Ca2+]i through T-type, voltage-operated calcium channels.
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