Effects of cryopreservation on the intracellular calcium concentration of human spermatozoa and its response to progesterone

@article{McLaughlin1994EffectsOC,
  title={Effects of cryopreservation on the intracellular calcium concentration of human spermatozoa and its response to progesterone},
  author={Eileen A. McLaughlin and William Christopher Liberty Ford},
  journal={Molecular Reproduction and Development},
  year={1994},
  volume={37}
}
The intracellular free calcium concentration [Ca2+]i of sperm from 23 ejaculates was measured before and after cryopreservation using the fluorescent probe Fura‐2. Spermatozoa were treated with 3.18 μM progesterone so that the regulation of [Ca2+]i in a dynamic situation could be studied. [Ca2+]i (nM) was 290 ± 13 in fresh spermatozoa vs. 550 ± 26 in cryopreserved samples (mean ± S.E.M. P < 0.0001 paired t‐test). Progesterone at a dose of 3.18 μM stimulated a large and rapid increase in [Ca2+]i… 
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