Effects of cryopreservation on sperm structure in Japanese pearl osyter Pinctada fucata martensii

@article{Narita2009EffectsOC,
  title={Effects of cryopreservation on sperm structure in Japanese pearl osyter Pinctada fucata martensii},
  author={Teruyoshi Narita and Takayuki Kawamoto and Kiyoshi Isowa and Hideo Aoki and Masahiro Hayashi and Hiromi Ohta and Akira Komaru},
  journal={Fisheries Science},
  year={2009},
  volume={74},
  pages={1069-1074}
}
To clarify factors reducing the motility and fertility of cryopreserved spermatozoa of the Japanese pearl oyster Pinctada fucata martensii, the structure of spermatozoa before and after cryopreservation was observed by scanning electron microscopy. Testicular spermatozoa were diluted with cryopreservation diluent (10% methanol+18% fetal bovine serum+72% sea water), and dispensed into 0.25-mL straws. The straws were cooled at a rate of approximately −20 °C/min to −50°C, and subsequently immersed… CONTINUE READING
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References

Publications referenced by this paper.
Showing 1-10 of 17 references

Lesions of spermatozoa by cryopreservation in oyster Crassostrea gigas

  • H Kurokura, K Namba, K. Ishikawa
  • Nippon Suisan Gakkaishi
  • 1990
Highly Influential
4 Excerpts

Studies on cryopreservation of sea urchin spermatozoa

  • H Kurokura, N Yagi, R. Hirano
  • Suisanzoshoku
  • 1989
Highly Influential
5 Excerpts

Fertility of cryopreservation spermatozoa of the Japanese pearl oyster, Pinctada fucata martensii

  • T Narita, T Kawamoto, +4 authors H. Ohta
  • Aquaculture
  • 2008
1 Excerpt

Cryopreservation of finfish and shellfish gametes and embryos

  • NH Chao, IC Liao
  • Aquaculture
  • 2001
2 Excerpts

Mass mortalities of Japanese pearl oyster in Uwa Sea, Ehime in 1997–1999

  • T Morizane, S Takimoto, +9 authors M. Ichikawa
  • Fish Pathol
  • 2001
1 Excerpt

Cryopreservation of aquatic invertebrate semen: a review

  • JC Gwo
  • Aquacult. Res
  • 2000
1 Excerpt

Experimental infections of a disease causing mass mortalities of Japanese pearl oyster Pinctada fucata martensii by tissue transplantation and cohabitation

  • T Kurokawa, T Suzuki, +7 authors S. Funakoshi
  • Nippon Suisan Gakkaishi
  • 1999
1 Excerpt

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