Corpus ID: 2744874

Effects of cryopreservation on Ca2+ signals induced by membrane depolarization, caffeine, thapsigargin and progesterone in boar spermatozoa.

@article{Kim2008EffectsOC,
  title={Effects of cryopreservation on Ca2+ signals induced by membrane depolarization, caffeine, thapsigargin and progesterone in boar spermatozoa.},
  author={Joon-Chul Kim and Yuhua Li and Sunwoo Lee and Y. Yi and Chang-Sik Park and S. Woo},
  journal={Molecules and cells},
  year={2008},
  volume={26 6},
  pages={
          558-65
        }
}
Although the fertilizing ability of spermatozoa is greatly reduced after freezing, complete understanding of alterations induced by cryopreservation has not been elucidated. The present study evaluates the effects of cryopreservation on the Ca2+ handling of boar spermatozoa using several sperm activators. Intracellular Ca2+ signals from single spermatozoa were measured using confocal Ca2+ imaging of unfrozen samples and of other spermatozoa after having been frozen. Elevation of the external K… Expand
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Evaluating the effects of cryopreservation on MPT and its relationship with the deterioration of sperm quality in a bovine model showed that thawed spermatozoa trigger MPT associated with an intracellular calcium increase and this was accompanied by ΔΨm dissipation, decrease of ATP levels and ROS production, and deterioration of plasma membrane integrity. Expand
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TLDR
The present work summarizes the principles of cryoinjury and the relevance of permeating and nonpermeating cryoprotective agents and speculates with new research horizons on the preservation of boar sperm, such as vitrification and freeze-drying. Expand
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It is concluded that cryopreservation causes a substantial elevation of the [Ca2+]i in human spermatozoa and damage to the plasma membrane during cryop Reservation may result in the loss of the progesterone receptor. Expand
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Cryopreservation procedures reduce the responsiveness of human spermatozoa to progesterone in terms of [Ca(2+)](i) rise and completely inhibit its effects on plasma membrane potential variations, thus supporting the hypothesis that freezing-thawing procedures may differently modify the plasma membrane receptors for progester one in human spermutozoa which are known to express at least two receptors for this steroid in their plasma membrane. Expand
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