Effects of cooling and warming rates during vitrification on fertilization of in vitro-matured bovine oocytes.

@article{Hochi2001EffectsOC,
  title={Effects of cooling and warming rates during vitrification on fertilization of in vitro-matured bovine oocytes.},
  author={Shinichi Hochi and Masayuki Akiyama and Gen Minagawa and Koji Kimura and Andrea Lika Hanada},
  journal={Cryobiology},
  year={2001},
  volume={42 1},
  pages={
          69-73
        }
}
The purpose of this study was to clarify the relationship of cooling rates (CR) and warming rates (WR) during vitrification with postwarming viability of in vitro-matured bovine oocytes. In Experiment 1, oocytes were vitrified in a solution containing 7.2 M ethylene glycol and 1.0 M sucrose by use of open-pulled glass capillaries with five different outer diameters and were warmed by placement of the capillaries into 0.25 M sucrose solution. The capillaries of 2000-, 1400-, 1000-, 630-, and 440… CONTINUE READING

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