Effects of cofilin on actin filamentous structures in cultured muscle cells. Intracellular regulation of cofilin action.

@article{Nagaoka1995EffectsOC,
  title={Effects of cofilin on actin filamentous structures in cultured muscle cells. Intracellular regulation of cofilin action.},
  author={Rie Nagaoka and Ken-ichi Kusano and Hiroshi Abe and Takashi Obinata},
  journal={Journal of cell science},
  year={1995},
  volume={108 ( Pt 2)},
  pages={581-93}
}
The previous investigation (Abe et al. (1989) J. Biochem. 106, 696-702) suggested that cofilin is deeply involved in the regulation of actin assembly in developing skeletal muscle. In this study, to examine further the function of cofilin in living myogenic cells in culture, recombinant cofilin having extra Cys residues at the N terminus was produced in Escherichia coli and was labeled with tetramethylrhodamine-iodoacetamide (IATMR). When the cofilin labeled with IATMR (IATMR-cofilin) was… CONTINUE READING

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