Effects of chymotrypsin and trypsin on rat peritoneal mast cells.

@article{Lagunoff1975EffectsOC,
  title={Effects of chymotrypsin and trypsin on rat peritoneal mast cells.},
  author={David Lagunoff and Emil Y. Chi and H Wan},
  journal={Biochemical pharmacology},
  year={1975},
  volume={24 17},
  pages={
          1573-8
        }
}
Abstract Alpha-chymotrypsin was demonstrated to release histamine from rat peritoneal mast cells. Release of histamine was shown by electron microscopy not to be cytotoxic. Release was temperature dependent, required Ca 2+ and cell ATP for optimal effect, and involved the direct action of α-chymotrypsin on the mast cells. Trypsin was far less active in releasing histamine, but exposure of the cells to trypsin inhibited the subsequent release of histamine by chymotrypsin. The relationship… CONTINUE READING