• Biology
  • Published 2008

Effects of chymase on proliferation and collagen synthesis of rat cardiac fibroblasts

@inproceedings{Xiaolong2008EffectsOC,
  title={Effects of chymase on proliferation and collagen synthesis of rat cardiac fibroblasts},
  author={Lu Xiao-long},
  year={2008}
}
AIM To investigate the effects of chymase on the proliferation and collagen synthesis of rat cardiac fibroblasts(CFs).METHODS Cultured CFs from neonatal SD rats were isolated by trypsinization and cellular number and cellular cycle were evaluated by MTT colorimetry(A490 value) and flow cytometry(FCM).The total collagen synthesis by CFs was examined by 3H-proline incorporation and the expressions of TypesⅠ and Ⅲ collagen mRNA in CFs were determined by RT-PCR.RESULTS ①Chymase increased CFs number… CONTINUE READING