Effects of choline deficiency and methotrexate treatment upon rat liver.
@article{Pomfret1990EffectsOC, title={Effects of choline deficiency and methotrexate treatment upon rat liver.}, author={Elizabeth Anne Pomfret and Kerry Ann daCosta and Steven H. Zeisel}, journal={The Journal of nutritional biochemistry}, year={1990}, volume={1 10}, pages={ 533-41 } }
72 Citations
Effects of choline deficiency and methotrexate treatment upon liver folate content and distribution.
- Chemistry, BiologyCancer research
- 1991
The effects of feeding rats a choline deficient diet, of treating rats with low doses of methotrexate, and of combined choline deficiency and MTX treatment upon the content and distribution of folates in liver were examined.
Choline deficiency and methotrexate treatment induces marked but reversible changes in hepatic folate concentrations, serum homocysteine and DNA methylation rates in rats.
- Medicine, BiologyJournal of the American College of Nutrition
- 1995
It is suggested that CD and MTX treatment appear to impair the capacity of tissues to incorporate folate in only 2 weeks and affect other biomarkers of one-carbon metabolism such as Hcy concentration and DNA methylation.
Effects of betaine supplementation and choline deficiency on folate deficiency-induced hyperhomocysteinemia in rats.
- BiologyJournal of nutritional science and vitaminology
- 2012
The effect of betaine status on folate deficiency-induced hyperhomocysteinemia was investigated to determine whether folate deficiency impairs homocysteine removal not only by the methionine synthase…
Methotrexate alters carbon flow through the hepatic folate-dependent one-carbon pool in rats.
- Chemistry, BiologyCarcinogenesis
- 1996
The tracer kinetic experiments quantitatively demonstrate the extent to which methotrexate alters the actual carbon flow through the hepatic folate-dependent one-carbon pool, primarily directed at diminishing the reductive carbon flow towards methyltetrahydrofolate and methionine synthesis.
Diethanolamine induces hepatic choline deficiency in mice.
- Medicine, BiologyToxicological sciences : an official journal of the Society of Toxicology
- 2002
It is demonstrated that DEA treatment causes a spectrum of biochemical changes consistent with choline deficiency in mice and a clear dose concordance between DEA-induced choline deficiencies and hepatocarcinogenic outcome is demonstrated.
Choline deprivation induces hyperhomocysteinemia in rats fed low methionine diets.
- BiologyJournal of nutritional science and vitaminology
- 2008
The results indicate that choline deprivation can easily induce prominent hyperhomocysteinemia when rats are fed relatively low methionine diets such as a standard soybean protein diet and low casein diet, possibly through the suppression of homocysteine removal by both remethylation and cystathionine formation.
MTX does not affect enhanced biosynthesis and metabolism of S-adenosylmethionine in testosterone-induced hypertrophic mouse kidney.
- Biology, MedicineAdvances in experimental medicine and biology
- 1993
One of the anabolic effects of testosterone, apart from its profound induction of ornithine decarboxylase, which results in the stimulation of putrescine biosynthesis, is the enhancement of synthesis and levels of S-adenosylmethionine (AdoMet), and an increase in cellular methylation intensity.
Genetic variation of folate-mediated one-carbon transfer pathway predicts susceptibility to choline deficiency in humans.
- Biology, MedicineProceedings of the National Academy of Sciences of the United States of America
- 2005
A strong association in premenopausal women of the 5,10-methylenetetrahydrofolate dehydrogenase-1958A gene allele polymorphism with 15 times increased susceptibility to developing organ dysfunction on a low-choline diet is found.
Phosphatidylethanolamine N-methyltransferase (PEMT) knockout mice have hepatic steatosis and abnormal hepatic choline metabolite concentrations despite ingesting a recommended dietary intake of choline.
- Biology, MedicineThe Biochemical journal
- 2003
The results show that PEMT normally supplies a significant portion of the daily choline requirement in the mouse and, when this pathway is knocked out, mice are unable to attain normal concentrations of all choline metabolites even with a supplemental source of dietary choline.
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The data strongly suggest that, in the rat model, methotrexate produced liver toxicity by virtue of an effect other than inhibition of DNA synthesis; and that this toxicity can be blocked without impairing metotrexate effect on bone marrow by the administration of choline, a lipotropic agent requiring methylation for its synthesis.
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Investigation of the effects of moderate dose to very high dose methotrexate on methionine and total homocysteine as reflections of metotrexate induced intracellular events found changes in homocy steine and methionines may reflect biological effects of methot Rexate that may predict cytotoxicity of metHotrexate.
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The effect of dietary choline upon liver ethanolamine metabolism was investigated by measurement of the changes in the liver levels of phospholipids and their precursors and in the metabolism of…