Effects of cell culture density on phagocytosis parameters in IC-21 macrophages

@article{Vishniakova2011EffectsOC,
  title={Effects of cell culture density on phagocytosis parameters in IC-21 macrophages},
  author={Kh. S. Vishniakova and Igor I Kireev and Antonina Dunina-Barkovskaya},
  journal={Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology},
  year={2011},
  volume={5},
  pages={355-363}
}
Cell culture density is shown to alter the parameters characterizing phagocytic activity of cells in vitro. Phagocytosis index (PI, mean number of beads per cell in the bead-containing population) and phagocytosis percent (PP, percentage of bead-containing cells in cell population under study) for IC-21 macrophages incubated in the presence of non-opsonized 2-μm fluorescent latex beads were determined using fluorescent microscopy and ImageJ software specially adapted for the purpose. Under… CONTINUE READING

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