Effects of capsaicin on nitric oxide synthase isoforms in prepubertal rat ovary

  title={Effects of capsaicin on nitric oxide synthase isoforms in prepubertal rat ovary},
  author={Berrin Zik and Korhan Altunbaş and Serife Tutuncu and Onder Ozden and CG Ozguden Akkoc and Sabire Peker and Alper Sevimli},
  journal={Biotechnic \& Histochemistry},
  pages={218 - 225}
Abstract Nitric oxide (NO) has emerged as an important intra-ovarian regulatory factor. We investigated effects of low dose capsaicin (CAP) treatment on the different NOS isoforms in prepubertal rat ovaries. Fifteen 21-day-old female Sprague-Dawley rats were divided randomly into three groups. The first group received no treatment, the second group received 0.5 mg/kg/day CAP dissolved in the vehicle, and the third group was treated with the vehicle only. The animals were euthanized by ether… 

The effect of capsaicin on inducible nitric oxide synthase in puberty and adult rats’ ovaries

The reaction density was reduced in the experimental groups and showed long-term capsaicin implementation in low dosage negatively affects iNOS stimulation in ovaries.

Grape seed proanthocyanidin extract alleviates urethral dysfunction in diabetic rats through modulating the NO-cGMP pathway.

It is demonstrated that GSPE protects urethra function in DM rats through modulating the NO-cGMP signaling pathway and may be associated with activation of the Nrf2 defense pathway.

Immunohistochemical distribution and gene expression of transforming growth factor alpha in ovarian tissue of rats treated with capsaicin in puberty

The results showed that capsaicin had no effects on ovaries at different developmental stages as well as immunohistochemically and histologically.

Matrix metalloproteinase, vitamin A and methylprednisolone effects on experimentally induced amyloid arthropathy

Vitamin A significantly enhanced the effect of vitamin A and the inhibiting effect of methylprednisolone on amyloid arthropathy in brown layer chicks and increased the release of MMP-9 in birds withAmyloidArthropathy.

Plant molecules and their influence on health and female reproduction



Hormonal Regulation of Nitric Oxide Synthases and Their Cell-Specific Expression during Follicular Development in the Rat Ovary1.

Rat ovaries were clearly devoid of brain NOS staining at any of the time points studied, and localize NOS isoforms in the rat ovary and to examine their hormonal regulation.

Hormonal regulation of nitric oxide synthases and their cell-specific expression during follicular development in the rat ovary.

It is demonstrated that ovarian eNOS and iNOS show distinct cell-specific expression patterns and are differentially regulated during follicular and luteal development.

Expression of nitric oxide synthase and effect of substrate manipulation of the nitric oxide pathway in mouse ovarian follicles.

NOS is present in mouse follicles, and its action is necessary at a local level for normal follicle development in vitro, where vascular effects of NO are attenuated.

Secretion from neuropeptide-treated splenocytes modifies ovarian steroidogenesis.

The ovarian steroidogenic response, which was modified by the effects of an in vivo SON transection on spleen cells, was reverted by an in vitro system in which the splenocytes were treated with VIP or NPY.

Abnormal estrous cyclicity after disruption of endothelial and inducible nitric oxide synthase in mice.

The dysfunction in cyclicity, ovulation rate, ovarian morphology, and steroidogenesis in eNOS-knockout female mice strongly supports the concept that eN OS/NO plays critical roles in ovulation and follicular development.

Nitric oxide inhibits steroidogenesis in cultured porcine granulosa cells.

It is demonstrated for the first time that NO inhibits oestradiol secretion independent of cGMP by inhibiting P450 aromatase activity in moderately mature PGC.

Cell-specific localization of nitric oxide synthases (NOS) in the rat ovary during follicular development, ovulation and luteal formation.

Immunohistochemical analysis using polyclonal eNOS and iNOS antibodies revealed the localization of these two isoforms primarily in the S and the T of the periovulatory ovary, suggesting a role for NO in the ovulatory process and in the regulation of CL function.

Nitric oxide: an autocrine regulator of human granulosa-luteal cell steroidogenesis.

It is concluded that NO synthase is present in human granulosa-luteal cells and that NO inhibits estradiol secretion independent of cGMP by directly inhibiting aromatase.

Expression and localization of inducible and endothelial nitric oxide synthase in the rat ovary. Effects of gonadotropin stimulation in vivo.

It is concluded that two isoforms of NOS are expressed in the ovary and the mRNA levels for these isozymes are differentially regulated.