Effects of ascorbic acid on in vitro culture of bovine preantral follicles

@article{Andrade2012EffectsOA,
  title={Effects of ascorbic acid on in vitro culture of bovine preantral follicles},
  author={Evelyn Rabelo Andrade and Robert van den Hurk and Livia Aires Lisboa and Mariana Fernandes Hertel and Fabiana de Andrade Melo-Sterza and Kleber Moreno and Ana Paula Bracarense and Fernanda da Cruz Landim‐Alvarenga and Marcelo Marcondes Seneda and Amauri Alcindo Alfieri},
  journal={Zygote},
  year={2012},
  volume={20},
  pages={379 - 388}
}
Summary The objective of this study was to evaluate the effects of adding ascorbic acid to the media for in vitro culture of cattle ovarian fragments and to determine their effects on growth activation and viability of early-stage follicles. The ovarian cortex was divided into small fragments; one fragment was immediately fixed (control) and the other fragments were cultured in minimum essential medium (MEM) supplemented or not with various doses of ascorbic acid. Ovarian tissue was processed… 
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20 Citations
Background Citations
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Methods Citations
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Results Citations
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20 Citations

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Citation Type

Improvement of development of equine preantral follicles after 6 days of in vitro culture with ascorbic acid supplementation.
Effects of epidermal growth factor and progesterone on development, ultrastructure and gene expression of bovine secondary follicles cultured in vitro.
Influence of follicle-stimulating hormone concentrations on the integrity and development of bovine follicles cultured in vitro
TLDR
It is concluded that the medium supplemented with 100 ng/ml FSH during in vitro culture provided appropriate conditions for the development and morphological integrity of preantral follicles in cattle.
Interval in the replacement of in vitro culture medium affects the integrity and development of equine preantral follicles
TLDR
There was no significant difference in relation to follicular integrity after medium replacement after in vitro culture of preantral follicles at 2 or 6 days, but changing the medium for equine species at 48 hours after the start of culture followed by subsequent daily replacements is suggested.
Alpha lipoic acid (ALA) effects on developmental competence of equine preantral follicles in short-term culture.
Early ovine preantral follicles have a potential to grow until antral stage in two-step culture system in the presence of aqueous extract of Justicia insularis.
TLDR
SF can be successfully isolated from sheep ovarian cortex after 7 days of culture and are capable of surviving and forming an antral cavity if cultured in vitro for an additional 6 days in the presence of 0.3 mg/ml J. insularis.
In vitro growth and development of ovarian follicles for fertility preservation
TLDR
The overall results indicate that the IVG of oocytes is a suitable tool to both assess the patency of fertility preservation systems and as a means to restore female fertility.
The development and integrity of equine pre‐antral follicles cultured in vitro with follicle‐stimulating hormone (FSH) supplementation
TLDR
In conclusion, the in vitro culture of abattoir-derived equine ovarian fragments presented better morphological integrity when supplemented with FSH for 6 days, in comparison with the MEM culture group.
In vitro culture of bovine preantral follicles: a review
TLDR
This review will highlight the basic aspects of bovine folliculogenesis by focusing on preantral follicles, the methods of harvesting preantrals, the main results from in vitro follicular culture during the last 20 years, and the potential candidate substances (basic supplements, growth factors, and hormones) for improving the efficiency ofIn vitro follicle growth.
Amburana cearensis leaf extract maintains survival and promotes in vitro development of ovine secondary follicles
TLDR
It was shown that 0.1 mg/ml of this extract, without supplementation, maintains follicular survival and promotes the development of ovine isolated secondary follicles in vitro and can be an alternative culture medium for preantral follicle development.
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References

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TLDR
This culture system was capable of supporting follicle differentiation over the 12 day culture period, and ascorbic acid maintains bovine follicle health and basement membrane remodelling in vitro.
Role of ascorbic acid in promoting follicle integrity and survival in intact mouse ovarian follicles in vitro.
TLDR
It is concluded that ascorbic acid is necessary for remodelling the basement membrane during follicular growth and that the ability of follicles to uptake ascorBic acid confers an advantage in terms of granulosa cell survival.
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TLDR
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TLDR
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TLDR
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