Effects of arginine on Polytomella parva growth, PII protein levels and lipid body formation

  title={Effects of arginine on Polytomella parva growth, PII protein levels and lipid body formation},
  author={Tatiana V. Lapina and Lidiya Yu Kochemasova and Karl Forchhammer and Elena V. Ermilova},
Main conclusionl-Arginine supports growth and resulted in increased PII signaling protein levels and lipid droplet accumulation in the colorless green alga Polytomella parva.AbstractPolytomella parva, a model system for nonphotosynthetic green algae, utilizes ammonium and several carbon sources, including ethanol and acetate. We previously reported that P. parva accumulates high amounts of arginine with the key enzyme of the ornithine/arginine biosynthesis pathway, N-acetyl-L-glutamate kinase… 
From Cyanobacteria to Archaeplastida: new evolutionary insights into PII signaling in the plant kingdom.
This review considers recent advances in understanding metabolic signaling by PII proteins of the plant kingdom and the PII signaling system of red algae has been identified as an evolutionary intermediate between that of Cyanobacteria and Chloroplastida.
Arginine-Dependent Nitric Oxide Generation and S-Nitrosation in the Non-Photosynthetic Unicellular Alga Polytomella parva
The data argue for NR-independent NO synthesis and its active role in S-nitrosation as an essential post-translational modification in P. parva.


The Synechococcus elongatus PII signal transduction protein controls arginine synthesis by complex formation with N‐acetyl‐l‐glutamate kinase
This communication identifies, for the first time, a receptor protein for signal perception from the PII signal transduction protein in the cyanobacterium Synechococcus elongatus, subjecting the key enzyme of arginine biosynthesis to global nitrogen control.
Polytomella spp. growth on ethanol. Extracellular pH affects the accumulation of mitochondrial cytochrome c550.
A defined medium with ethanol as sole carbon source was devised for growth of the colorless, unicellular alga Polytomella spp. Cell density on this carbon source was related to extracellular pH. An
Polytomella spp. growth on ethananol
The participation of cytochrome c550 in mitochondrial electron transport from succinate to oxygen was shown by spectral measurements, and RNA blot analysis gave clear evidence of the abundance of c550 transcript in cells grown at pH 3.7.
The PII signaling protein from red algae represents an evolutionary link between cyanobacterial and Chloroplastida PII proteins
The results highlight the PII signaling system in red algae as an evolutionary intermediate between Cyanobacteria and Chlorophyta and emphasize the conserved role of NAGK as a major PII-interactor throughout the evolution of oxygenic phototrophs.
Interaction of the Nitrogen Regulatory Protein GlnB (PII) with Biotin Carboxyl Carrier Protein (BCCP) Controls Acetyl-CoA Levels in the Cyanobacterium Synechocystis sp. PCC 6803
The PII protein of a unicellular cyanobacterium inhibits the biosynthetic activity of E. coli ACC and also interacts with cyanobacterial BCCP in an ATP and 2-oxoglutarate dependent manner and in a PII mutant strain of Synechocystis strain PCC 6803, the lacking control leads to reduced acetyl-CoA levels.
Interaction of N‐acetyl‐l‐glutamate kinase with the PII signal transducer in the non‐photosynthetic alga Polytomella parva: Co‐evolution towards a hetero‐oligomeric enzyme
Polytomella parva has lost the ability to estimate the cellular energy and carbon status but has specialized to provide an entirely glutamine‐dependent arginine feedback control, highlighting the evolutionary plasticity of PII signalling system.