Effects of ammonium and lactate on growth and metabolism of a recombinant Chinese hamster ovary cell culture.

@article{Lao1997EffectsOA,
  title={Effects of ammonium and lactate on growth and metabolism of a recombinant Chinese hamster ovary cell culture.},
  author={M S Lao and Derek Toth},
  journal={Biotechnology progress},
  year={1997},
  volume={13 5},
  pages={688-91}
}
A Chinese hamster ovary (CHO) cell line producing a recombinant glycoprotein was cultured in batch mode with different initial concentrations of ammonium chloride (0-10 mM), sodium lactate (0-60 mM), or sodium chloride (0-60 mM). High ammonium concentrations did not inhibit cell growth and productivity or glucose and glutamine consumption. In contrast, specific ammonia and alanine production decreased by 55% and 40%, respectively. There were also significant increases in specific aspartate and… CONTINUE READING