Effects of amino acids on Thiobacillus acidophilus. II. Threonine deaminase.

@article{Proteau1980EffectsOA,
  title={Effects of amino acids on Thiobacillus acidophilus. II. Threonine deaminase.},
  author={G. Proteau and M. Silver},
  journal={Canadian journal of microbiology},
  year={1980},
  volume={26 3},
  pages={
          385-8
        }
}
Biosynthetic L-threonine deaminase was partially purified 73-fold with a 60% recovery from Thiobacillus acidophilus by ammonium sulfate fractionation and by Sepharose 6B-C1 chromatography. The optimal pH for enzyme activity was between 9.0 and 10.0 and no optimal pH shift was observed in the presence of L-isoleucine, an inhibitor. The enzyme was effectively inhibited by L-isoleucine and showed homotropic interaction only in the presence of L-isoleucine. Kinetic studies indicate that there are… Expand
A systems approach to model natural variation in reactive properties of bacterial ribosomes