Effects of aging, dietary restriction and glucocorticoid treatment on housekeeping gene expression in rat cortex and hippocampus—Evaluation by real time RT-PCR

@article{Tani2007EffectsOA,
  title={Effects of aging, dietary restriction and glucocorticoid treatment on housekeeping gene expression in rat cortex and hippocampus—Evaluation by real time RT-PCR},
  author={Nikola T. Tani{\'c} and Milka Perovic and Aleksandra Mladenovi{\'c} and Sabera D Ruzdiji{\'c} and Selma Kanazir},
  journal={Journal of Molecular Neuroscience},
  year={2007},
  volume={32},
  pages={38-46}
}
Accurate normalization is the prerequisite for obtaining reliable results in the quantification of gene expression. Using TaqMan Real Time RT-PCR, we carried out an extensive evaluation of five most commonly used endogenous controls, gapdh, β-actin, 18S rRNA, hprt and cypB, for their presumed stability of expression, in rat cortex and hippocampus, during aging, under dietary restriction and dexamethasone treatment. Valid reference genes (HKGs) were identified using GeNorm and Norm-Finder… CONTINUE READING

From This Paper

Figures, tables, results, connections, and topics extracted from this paper.
34 Extracted Citations
33 Extracted References
Similar Papers

Citing Papers

Publications influenced by this paper.
Showing 1-10 of 34 extracted citations

Referenced Papers

Publications referenced by this paper.
Showing 1-10 of 33 references

Similar Papers

Loading similar papers…