Effects of adenosine dialdehyde treatment on in vitro and in vivo stable protein methylation in HeLa cells.

@article{Chen2004EffectsOA,
  title={Effects of adenosine dialdehyde treatment on in vitro and in vivo stable protein methylation in HeLa cells.},
  author={Da-Huang Chen and K Wu and Chien-Jen Hung and Mingli Hsieh and Chuan Li},
  journal={Journal of biochemistry},
  year={2004},
  volume={136 3},
  pages={371-6}
}
Adenosine dialdehyde (AdOx) is an indirect methyltransferase inhibitor broadly used in cell culture to accumulate methyl-accepting proteins in hypomethylated states for in vitro protein methylation analyses. In this study we included a translation inhibitor, cycloheximide, in the AdOx treatment of HeLa cells. The methyl-accepting proteins disappeared in the double treatment, indicating that they were most likely newly synthesized in the AdOx incubation period. AdOx treatment could also be used… CONTINUE READING