Effects of adenosine 5'-triphosphate and growth hormone on cellular H+ transport and calcium ion concentrations in cloned bovine mammary epithelial cells.

@article{Katoh2001EffectsOA,
  title={Effects of adenosine 5'-triphosphate and growth hormone on cellular H+ transport and calcium ion concentrations in cloned bovine mammary epithelial cells.},
  author={Kazuo Katoh and Tokushi Komatsu and Shinichi Yonekura and Hiroko Ishiwata and Akihiko Hagino and Yoshiaki Obara},
  journal={The Journal of endocrinology},
  year={2001},
  volume={169 2},
  pages={381-8}
}
The present experiment was carried out to investigate the effects of exogenous adenosine 5'-triphosphate (ATP) and growth hormone (GH) on cellular H(+) efflux rate (extracellular acidification rate) and Ca(2+) concentration ([Ca(2+)](c)) in cloned bovine mammary epithelial cells (bMEC) raised from the mammary gland of a 26-day-pregnant Holstein heifer. Perifusion of 2-day cultured cells with a medium containing ATP (10, 100 and 1000 micromol/l) for 30 min caused a significant and concentration… CONTINUE READING
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