Effects of acute alcohol intoxication on gluconeogenesis and its hormonal responsiveness in isolated, perfused rat liver.

@article{Deaciuc1992EffectsOA,
  title={Effects of acute alcohol intoxication on gluconeogenesis and its hormonal responsiveness in isolated, perfused rat liver.},
  author={Ion V Deaciuc and N B D'souza and Charles H Lang and John J. Spitzer},
  journal={Biochemical pharmacology},
  year={1992},
  volume={44 8},
  pages={1617-24}
}
Rats were acutely administered ethanol as a primed constant infusion in order to produce sustained blood ethanol levels of 8-12 or 55-65 mM. At the end of ethanol infusion the livers were either freeze-clamped in vivo or isolated and perfused for metabolic studies. The rate of gluconeogenesis and its responsiveness to phenylephrine (10 microM), prostaglandin F2 alpha (5 microM) and glucagon (10 nM), as well as the redox state of the cytosolic NAD(+)-NADH system were assessed in livers isolated… CONTINUE READING

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