Effects of Two Different Digestion Methods on Culture of Mouse Mammary Epithelial Cells

@inproceedings{Han2014EffectsOT,
  title={Effects of Two Different Digestion Methods on Culture of Mouse Mammary Epithelial Cells},
  author={Yang Han},
  year={2014}
}
In order to explore the enzyme digestion on mouse mammary epithelial cells(MECs)cultureed in vitro,the mammary tissue was isolated from mice aseptically in vitro,collagenase/trypsin digestion and two-step enzyme digestion were used to culture mouse MECs.The keratin 18,a specific marker for epithelial cell,was identifiedby confocal lasers scanning microscope.The results showed that plenty of pure mouse MECs can be harvested by using collagenase/trypsin digestion and continuously cultured for… CONTINUE READING