Effects of Thyrotropin-releasing Hormone and Hydrocortisone on Synthesis and Degradation of Prolactin in a Rat Pituitary Cell Strain *

  • Published 2002


Synthesis of prolactin was measured in a clonal strain of functional pituitary cells in culture by incubating the cells with [sH]leucine; labeled prolactin was separated from the other labeled proteins by specific immunoprecipitation followed by dissociation with sodium dodecyl sulfate and disc gel electrophoresis. Accumulation of labeled prolactin in the… (More)


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