Effects of TGF-beta3 and preculture period of osteogenic cells on the chondrogenic differentiation of rabbit marrow mesenchymal stem cells encapsulated in a bilayered hydrogel composite.

@article{Guo2010EffectsOT,
  title={Effects of TGF-beta3 and preculture period of osteogenic cells on the chondrogenic differentiation of rabbit marrow mesenchymal stem cells encapsulated in a bilayered hydrogel composite.},
  author={Xuan Guo and Jiehong Liao and Hansoo Park and Amit Saraf and Robert M. Raphael and Yasuhiko Tabata and F. Kurtis Kasper and Antonios G Mikos},
  journal={Acta biomaterialia},
  year={2010},
  volume={6 8},
  pages={2920-31}
}
In this work, injectable, biodegradable hydrogel composites of crosslinked oligo(poly(ethylene glycol) fumarate) and gelatin microparticles (MPs) were used to fabricate a bilayered osteochondral construct. Rabbit marrow mesenchymal stem cells (MSCs) were encapsulated with transforming growth factor-beta3 (TGF-beta3)-loaded MPs in the chondrogenic layer and cocultured with cells of different periods of osteogenic preculture (0, 3, 6 and 12 days) in the osteogenic layer to investigate the effects… CONTINUE READING