Effects of Supplemental Calcium or Calcium-binding Agents on Staphylococcal Bacteriophage Proliferation in Skim Milk.

@article{Das1967EffectsOS,
  title={Effects of Supplemental Calcium or Calcium-binding Agents on Staphylococcal Bacteriophage Proliferation in Skim Milk.},
  author={Nirmal K. Das and R T Marshall},
  journal={Applied microbiology},
  year={1967},
  volume={15 6},
  pages={1479-82}
}
Additions of 0.0005 N calcium borogluconate to Trypticase Soy Broth (TSB) produced an increase in phage titer about 1 million-fold, whereas its addition to skim milk resulted in about a 100-fold decrease in the maximal titer. Supplemental calcium had a stimulatory influence on bacterial growth in TSB but not in skim milk. Studies were made of the effect of binding of calcium of skim milk on the proliferation of staphylococcal bacteriophage. Sequestering the calcium with 2% phosphate mixture… CONTINUE READING