Effects of Some Organic Nitrogen Sources and Antibiotics on Callus Growth of Indica Rice Cultivars

  title={Effects of Some Organic Nitrogen Sources and Antibiotics on Callus Growth of Indica Rice Cultivars},
  author={Arman Pazuki and Jafar Asghari and Mohammad Mehdi Sohani and Mohammad Pessarakli and Fatemeh Aflaki},
  journal={Journal of Plant Nutrition},
  pages={1231 - 1240}
A factorial experiment based on a completely randomized design with three replicates was conducted to evaluate effects of sixteen different mixtures of organic nitrogen sources and antibiotics which were supplemented to an Murashige and Skoog (MS) medium containing 4 mg L−1 2,4-Dichlorophenoxyacetic acid (2,4-D), 0.4 mg L−1 benzyl-aminopurine (BAP), 30 g L−1 sucrose, 8 g L−1 Agar-agar on fresh weight and dry weight (assessed after three days of drying at 70°C) of calli and in vitro callogenesis… 
Effect of some of the natural organic sources on rice tissue culture
Objective The main goal of this investigation was to evaluate the effect of glutamine, tryptophan, and casein hydrolysate on callus induction and shoot regeneration in two Egyptian rice cultivars
The development salt-tolerant sesame lines that have the resilient to the effects of climate changes are becoming an urgent need. The screening focuses on the response of 23 lines of sesame grown in
Evaluating Foliar Application of Calcium Nitrate, Calcium Chloride and Boric Acid on Physiological Disorders of ‘Kaleh - Ghoochi’ Pistachio
Pistachio suffers from some physiological disorders such as fruit abscission, endocarp lesion, blankness, non-split, early-split and deformed nuts. In this experiment, the effects of calcium nitrate
Effective Screening of Transgenic Pigeonpea in Presence of Negative Selection Agents
Positive transformants survived in both the selection processes, were further confirmed by polymerase chain reaction analysis and will facilitate the incorporation of agronomically important traits in transgenic pigeonpea for its betterment.


Effect of sorbitol concentration on regeneration of embryogenic calli in upland rice varieties (Oryza sativa L.)
This study describes the impact of sorbitol on plantlets regeneration frequency (PRF) of four rice cultivars (japonica, Oryza sativa L.) both of which mature and immature embryo-derived calli were investigated and appeared to be the most responsive genotype compared to other cultivars in PRF.
Establishment of a high efficiency Agrobacterium-mediated transformation system of rice (Oryza sativa L.).
  • K. Ozawa
  • Biology, Medicine
    Plant science : an international journal of experimental plant biology
  • 2009
Effects of Antibiotics on Suppression of Agrobacterium tumefaciens and Plant Regeneration from Wheat Embryo
Timentin can be used as an effective antibiotic in Agrobacterium-mediated wheat transformation and was higher in the treatment with timentin than with cefotaxime and clavamox at 500 mg/l of concentration.
The toxicity of antibiotics and herbicides on in vitro adventitious shoot formation of Pinus pinea L. cotyledons
Phosphinothricin and its ammonium salt glufosinate in the commercial formulation Finale® proved to be the best selection agents because they were less toxic to stone pine cotyledons and could be applied immediately after transformation to avoid escapes.
High efficiency genetic transformation of sour orange (Citrus aurantium) and production of transgenic trees containing the coat protein gene of citrus tristeza virus.
In preliminary experiments on Agrobacterium-mediated transformation of citrus, we found transformation events occurring in callus formed from the cambium. Factors affecting Agrobacterium-sour orange
Effects of Osmoprotectants upon NaCl Stress in Rice
It is indicated that during osmotic stress trehalose or carbohydrates might be more important for rice than Pro, which either has no effect or exasperates the effect of NaCl on growth inhibition, chlorophyll loss, and induction of a highly sensitive marker for plant stress, the osmotically regulated salT gene.
A tissue culture system for different germplasms of indica rice
Establishment of a highly efficient and widely used tissue culture system for indica rice will accelerate the application of transformation technology in breeding programs and the study of the functions of indica-specific genes.
Japonica rice varieties (Oryza sativa, Nipponbare, and others).
A rapid and user-friendly protocol based on mature seeds that can deliver transgenic rice plant-lets within 2 mo and validated the robustness and reliability at a high-throughput scale for several japonica genotypes.
Improved protocols for transformation of indica rice mediated by Agrobacterium tumefaciens
A highly efficient gene transfer method mediated by Agrobacterium tumefaciens was developed for Group I indica rice, which had been quite recalcitrant in tissue culture and transformation. Freshly