Effects of Site-specific Mutagenesis of K253 and N184V on the Thermostability of D-Glucose Isomerase.

Abstract

In order to enhance the thermostability of D-Glucose isomerase (GI), its mutants, GIK253R and GIN184V, were obtained with the double primer method of site-directed mutagenesis. Then their biochemical properties were assayed and compared with wild-type GI. The results showed that: (1) The mutant K253R was less stable than the wild-type GI at 70 degrees and… (More)

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@article{Wu1996EffectsOS, title={Effects of Site-specific Mutagenesis of K253 and N184V on the Thermostability of D-Glucose Isomerase.}, author={Chuan-jin Wu and Ya-zhong Xiao and Fan Long and Mai-kun Teng and Chun Wang and Tao Cui}, journal={Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica}, year={1996}, volume={28 3}, pages={272-278} }