Corpus ID: 87239554

Effects of RPB5-mediating Protein(RMP) on Cell Cycle of Hepatoma Cells

@article{Wenxiang2011EffectsOR,
  title={Effects of RPB5-mediating Protein(RMP) on Cell Cycle of Hepatoma Cells},
  author={Wei Wen-xiang},
  journal={China Biotechnology},
  year={2011}
}
Obiective:To study the effects and mechanisms of RMP on cell cycle of human hepatoma cells SMMC-7721.Methods:Construct the shRNA recombinant eukaryotic expression vector pGPU6-Neo-RMP.Establish shRNA RMP stable cell lines;RT-PCR was used to detect the interference efficacy against RMP gene;Flow cytometry was applied to measure the effect of RMP on cell cycle of SMMC-7721,and then the expressions of related genes of cell cycle were detected by RT-PCR.Results: The shRNA recombinant eukaryotic… Expand