Effects of P2-purinoceptor antagonists on ecto-nucleotidase activity of guinea-pig vas deferens cultured smooth muscle cells.


Ecto-nucleotidase activity was studied on primary cultures of guinea-pig vas deferens smooth muscle cells by measuring the inorganic phosphate (Pi) production using ATP as a substrate. The ecto-nucleotidase was insensitive to ouabain, oligomycin, sodium azide, p-nitrophenyl phosphate and B-glycerophosphate. Enzyme activity was highly dependent on either Ca2… (More)


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