Effects of NMDA and ferrous sulfate on oxidation and cell death in primary neuronal cultures

@article{Rudolph2000EffectsON,
  title={Effects of NMDA and ferrous sulfate on oxidation and cell death in primary neuronal cultures},
  author={Joseph G. Rudolph and John J. Lemasters and Fulton T Crews},
  journal={Neurochemistry International},
  year={2000},
  volume={37},
  pages={497-507}
}
Excessive oxidative radical production has been implicated in a variety of neurodegerative processes including NMDA (N-methyl-D-aspartate) mediated excitotoxicity. To determine the relationship of oxidation to NMDA-receptor mediated neuronal death, we exposed rat primary cortical neuronal cultures to ferrous sulfate and the fluorescent dyes dichlorofluorescin diacetate (H(2)DCF) and propidium iodide (PI) to monitor reactive oxygen species (ROS) and cell death, respectively in the same cultures… CONTINUE READING
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