Effects of N-glycosylation on in vitro activity of Bowes melanoma and human colon fibroblast derived tissue plasminogen activator.

@article{Wittwer1989EffectsON,
  title={Effects of N-glycosylation on in vitro activity of Bowes melanoma and human colon fibroblast derived tissue plasminogen activator.},
  author={Arthur J. Wittwer and Susan Carol Howard and Lawrence Silas Carr and N K Harakas and Joseph Feder and Raj B. Parekh and Pauline M. Rudd and Raymond A. Dwek and Thomas W. Rademacher},
  journal={Biochemistry},
  year={1989},
  volume={28 19},
  pages={7662-9}
}
Tissue-type plasminogen activator (t-PA), when isolated from human colon fibroblast (hcf) cells, is N-glycosylated differently than when isolated from the Bowes melanoma (m) cell line (Parekh et al., 1988). Both hcf- and m-t-PA can be separated into type I t-PA (with three occupied N-glycosylation sequons, at Asn-117, -184, and -448) and type II t-PA (with… CONTINUE READING