Effects of Mutagenesis in vitro on the Ability of Cloned Tomato Golden Mosaic Virus DNA to Infect Nicotiana benthamiana Plants

@inproceedings{Brough1988EffectsOM,
  title={Effects of Mutagenesis in vitro on the Ability of Cloned Tomato Golden Mosaic Virus DNA to Infect Nicotiana benthamiana Plants},
  author={Clare L. Brough},
  year={1988}
}
Insertion mutations introduced in vitro into cloned DNA of tomato golden mosaic virus that considerably shortened the length of the open reading frames (ORFs) ALl, AL2/AL3, BL1 or BRI, abolished the ability of the DNA to infect Nicotiana benthamiana seedlings. Mutants in which ORF AR1 was similarly shortened by an insertion or a 28 bp deletion were infectious, showing that the formation of coat protein or virions is not required for replication and systemic spread of virus DNA, although the… CONTINUE READING

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