Effects of Mg2+ and the beta gamma-subunit complex on the interactions of guanine nucleotides with G proteins.

Abstract

Mg2+ interacts with the alpha subunits of guanine nucleotide-binding regulatory proteins (G proteins) in the presence of guanosine-5'-[gamma-thio]triphosphate (GTP-gamma S) to form a highly fluorescent complex from which nucleotide dissociates very slowly. The apparent Kd for interaction of G alpha X GTP gamma S with Mg2+ is approximately 5 nM, similar to… (More)

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Cite this paper

@article{Higashijima1987EffectsOM, title={Effects of Mg2+ and the beta gamma-subunit complex on the interactions of guanine nucleotides with G proteins.}, author={Takanori Higashijima and Kathryn M. Ferguson and Paul C. Sternweis and Murray Smigel and Alfred G. Gilman}, journal={The Journal of biological chemistry}, year={1987}, volume={262 2}, pages={762-6} }