Effects of L‐asparaginase on protein and glycoprotein synthesis

@article{Kessel1970EffectsOL,
  title={Effects of L‐asparaginase on protein and glycoprotein synthesis},
  author={David Kessel and H. Bruce Bosmann},
  journal={FEBS Letters},
  year={1970},
  volume={10}
}
The anti-neoplastic enzyme L-asparaginase inhibits the growth of certain malignant cells by interference with cell nutrition [ 1,2] . We have recently reported [3] that Lasparaginase inhibits the incorporation of labeled L-fucose and D-glucosamine into glycoproteins of L5 178Y murine leukemia cells. Three questions arise with respect to this finding: 1) Is the inhibition of glycoprotein synthesis a consequence of a general inhibition of all protein formation? 2) Can the observed results be… Expand
A rapid and efficient method of radioactively labeling L‐asparaginase
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A method is described for the production of radioactively labeled ASNase, an enzyme that hydrolyzes the amide group of asparagine that inhibits the growth of certain neoplasms and may have early effects on the cell plasma membrane topography, fluidity, and electrokinetic properties. Expand
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  • Biology, Medicine
  • Cancer research
  • 1972
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The results suggest that the methylation of tRNA in murine leukemic cells may be modulated in cells with drug-resistant properties. Expand
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Abstract The electrophoretic mobility of L5178Y cells in 0.0145 M NaCl, 4.5% sorbitol, 0.6 mM NaHCO 3 , pH 7.2, at 25°C was — 1.78 μ·s −1 ·V −1 ·cm −1 while that of an L -asparaginase resistantExpand
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L-Asparaginase (L-asparagine amidohydrolase, E.C.3.5.1.1) catalyzes the hydrolysis of L-asparagine to yield L-aspartic acid and ammonia. The enzyme was first reported by Lang (1904), who demonstratedExpand
Asparaginyl-transfer RNA: a substrate for L-asparaginase.
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  • Biology, Medicine
  • Biochimica et biophysica acta
  • 1971
Abstract The enzyme l -asparaginase (E.C. 3.5.1.1) could convert asparaginyl-tRNAAsn to aspartyl-tRNAAsn, although at a rate slower by a factor of 2.5 · 107 than the analogous conversion of lExpand
Haemostatic alterations induced by treatment with asparaginases and clinical consequences.
TLDR
Therapeutic or prophylactic heparin doses are only partially effective, and direct thrombin or factor Xa inhibitors could play significant roles in the near future. Expand
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