Effects of IGFBP-2 overexpression in vitro and in vivo

  title={Effects of IGFBP-2 overexpression in vitro and in vivo},
  author={Eckhard Wolf and Harald Lahm and M. Wu and R. Wanke and Andreas Hoeflich},
  journal={Pediatric Nephrology},
Abstract Insulin-like growth factor-binding proteins (IGFBPs) are important modulators of IGF actions and may have both stimulatory and inhibitory effects. Expression of IGFBP-2 is increased after fasting and in a variety of pathological conditions. However, the specific role of IGFBP-2 in growth physiology remains to be determined. In this review, we summarize data from in vitro and in vivo models suggesting that IGFBP-2 has mainly inhibitory effects on IGF actions. Since the growth hormone… 
Differential effects of insulin-like growth factor binding proteins-1, -2, -3, and -6 on cultured growth plate chondrocytes.
The biological effects of IGFBP-1, -2, -3, and -6 on cultured growth plate chondrocytes that express the type 1 IGF receptor were investigated to investigate the potential importance of the IGFBPs as modulators of longitudinal growth in pediatric CRF.
Roles of insulin-like growth factor binding protein-2 (IGFBP-2) in glioblastoma.
The IGF-dependent and -independent functions of IGFBP-2 are outlined and the roles of IGF BP-2 in the progression of GBM are focused on.
Insulin-Like Growth Factor Binding Proteins in Kidney Disease
The role of IGFBPs in regulating transcription, inducing cell migration and apoptosis is closely related to the occurrence and development of kidney disease and IGFBP-7 has been used in clinical practice as a biomarker for early diagnosis and prognosis of AKI.
Growth Inhibition in Giant Growth Hormone Transgenic Mice by Overexpression of Insulin-Like Growth Factor-Binding Protein-2.
IGFBP-2 levels were highest in pancreas, followed by skeletal muscle, heart, kidney, brain,skin, skin, and spleen, and no elevation of IGF BP-2 wa... no elevation in tissue IGFBP- 2 levels between B and GB mice.
Interaction of Insulin-Like Growth Factor-Binding Protein 2 with α2-Macroglobulin in the Circulation
Results showed that IGFBP-2 associates with α2-macroglobulin (α2M), a protease inhibitor, which most likely contributes to the regulation of IGF BP-2 proteolysis and, thus, the activity of IGFs.
Growth inhibition in giant growth hormone transgenic mice by overexpression of insulin-like growth factor-binding protein-2.
This study demonstrates, for the first time, the potential of IGFBP-2 to inhibit GH-stimulated growth in giant transgenic mice, providing further evidence for an inhibitory effect of this IGFBP in vivo.
Free insulin-like growth factors -- measurements and relationships to growth hormone secretion and glucose homeostasis.
  • J. Frystyk
  • Biology, Medicine
    Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society
  • 2004
Potentiating role of IGFBP-2 on IGF-II-stimulated alkaline phosphatase activity in differentiating osteoblasts.
The results showed that a significant increase in AP expression was induced by IGF-II added to the differentiating osteoblasts continuously or in S1 but not in S2 or S3, and suggest that IGFBP-2, at nearly equimolar concentration with IGF- II, plays a potentiating role in IGF-ii action on ROB differentiation in vitro.
Growth hormone, insulin-like growth factor-1, and the kidney: pathophysiological and clinical implications.
This review focuses on the renal actions of GH and IGF-1, taking into account major advances in renal physiology and hormone biology made over the last 20 years, to move the understanding of GH/IGF-1 regulation of renal functions from a cellular to a molecular level.


Overexpression of insulin-like growth factor-binding protein-2 in transgenic mice reduces postnatal body weight gain.
The data suggest that IGFBP-2 represents a negative regulator of postnatal growth in mice, potentially by reducing the bioavailability of IGF-I.
Consequences of postnatally elevated insulin-like growth factor-II in transgenic mice: endocrine changes and effects on body and organ growth.
Elevated IGF-II in postnatal life has multiple endocrine consequences and subtle time-specific effects on organ growth, and is a major regulator of IGFBP-2.
Overexpression of insulin-like growth factor-binding protein-2 results in increased tumorigenic potential in Y-1 adrenocortical tumor cells.
Elevated levels of IGFBP-2 may contribute to the highly malignant phenotype of adrenocortical cancer by a thus far unknown, presumably IGF-independent, mechanism.
Expression of IGF-II and IGF binding proteins in differentiating human intestinal Caco-2 cells.
Results indicate that the expression of IGF-II, IGF BP-2, and IGFBP-6 is regulated in a differentiation-dependent manner in Caco-2 cells.
Expression of IGFBP-2, -3, and -4 mRNA during differentiation of Caco-2 colon epithelial cells.
It is hypothesize that IGFBP-3 and -4 are related to differentiation of Caco-2 cells, whereas IGF BP-2 is related to proliferation in Caco -2 cells.
Role of Insulin-Like Growth Factor Binding Protein-2 and Its Limited Proteolysis in Neuroblastoma Cell Proliferation: Modulation by Transforming Growth Factor-β and Retinoic Acid.
Investigation of the influence of the plasmin system, transforming growth factor-β and retinoic acid on cell growth and the IGF system using neuroblastoma cells found an increase in IGFBP-2 proteolysis resulted in a 5-fold loss of affinity for IGF-II.
Insulin-like growth factor binding proteins-2 and -3 stimulate growth hormone receptor binding and mitogenesis in rat osteosarcoma cells.
Results show that IGF-I and -II on the one hand and IGFBP-2 and -3 on the other hand exert opposite actions on [125I]hGH binding, IGFBP -2 and-3 exerting probably an IGF-independent effect.
Insulin-like growth factor (IGF)-binding proteins inhibit the smooth muscle cell migration responses to IGF-I and IGF-II.
In summary, IGF-I and -II stimulate smooth muscle cell migration after wounding, and this migratory response is modulated by IGFBPs, which appears to neutralize the effects of the IGFs by inhibiting their interaction with IGF receptors.
Role of insulin-like growth factor binding protein-2 and its limited proteolysis in neuroblastoma cell proliferation: modulation by transforming growth factor-beta and retinoic acid.
IGF-II mediates autocrine proliferation in neuroblastoma cells under the control of IGFBPs secreted by the cells, its bioavailability being enhanced as a result of plasmin-induced IGFBP-2 proteolysis.