• Corpus ID: 33495068

Effects of Hesperethusa Crenulata ’ s Bark Extract on Production of ProCollagen Type I and Inhibition of MMP-1 in Fibroblasts Irradiated UVB

@inproceedings{Amornnopparattanakul2012EffectsOH,
  title={Effects of Hesperethusa Crenulata ’ s Bark Extract on Production of ProCollagen Type I and Inhibition of MMP-1 in Fibroblasts Irradiated UVB},
  author={Paveena Amornnopparattanakul and Nantaka Khorana and Jarupa Viyoch},
  year={2012}
}

• Paveena Amornnopparattanakul, N. Khorana, J. Viyoch
• Published 2012
• Biology

Ultraviolet B (UVB) radiation induces photoaging by upregulating the expression of matrix metalloproteinase (MMP) and decreasing collagen synthesis in human skin cells. This study evaluated the effects of Hesperethusa crenulata’s bark extract on cytotoxicity and phototoxicity in human skin cells, MMP-1 and type I pro-collagen levels in UVB-irradiated human skin fibroblasts by ELISA. Powdered H.crenulata extracted with dichloromethane. The toxicity showed in term of percentage of cell viability and the morphology of cells was observed under microscope. Percent of cell viability was compared with control (non-treat). The results indicated that this extract didn’t show both cytotoxicity in human skin fibroblasts and phototoxicity in human skin keratinocytes at the range of dose that used in this study. For morphology, both of cells didn’t change after were treated with this extract. It was found that H.crenulata extract reduced MMP-1 expression and increased type I pro-collagen production. Keywords—Hesperethusa crenulata, pro-collagen type I, MMP1, fibroblasts irradiated UVB.