Effects of Glia Maturation Factor Overexpression in Primary Astrocytes on MAP Kinase Activation, Transcription Factor Activation, and Neurotrophin Secretion

@article{Zaheer2004EffectsOG,
  title={Effects of Glia Maturation Factor Overexpression in Primary Astrocytes on MAP Kinase Activation, Transcription Factor Activation, and Neurotrophin Secretion},
  author={Asgar Zaheer and Mark A. Yorek and Ram{\'o}n Lim},
  journal={Neurochemical Research},
  year={2004},
  volume={26},
  pages={1293-1299}
}
Using the replication-defective adenovirus vector, we overexpressed rat glia maturation factor (GMF) in primary astrocyte cultures derived from embryonic rat brains. Among the three isoforms of MAP kinase, there was a big increase in the phosphorylation of p38, as detected with Western blotting using the phosphospecific antibody. Likewise, there was a substantial increase in the phosphorylation of the transcription factor CREB. Using the electrophoretic mobility shift assay (EMSA), we found a… 
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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